Unknown

Dataset Information

0

High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo.


ABSTRACT: The ability to probe the membrane potential of multiple genetically defined neurons simultaneously would have a profound impact on neuroscience research. Genetically encoded voltage indicators are a promising tool for this purpose, and recent developments have achieved a high signal-to-noise ratio in vivo with 1-photon fluorescence imaging. However, these recordings exhibit several sources of noise and signal extraction remains a challenge. We present an improved signal extraction pipeline, spike-guided penalized matrix decomposition-nonnegative matrix factorization (SGPMD-NMF), which resolves supra- and subthreshold voltages in vivo. The method incorporates biophysical and optical constraints. We validate the pipeline with simultaneous patch-clamp and optical recordings from mouse layer 1 in vivo and with simulated and composite datasets with realistic noise. We demonstrate applications to mouse hippocampus expressing paQuasAr3-s or SomArchon1, mouse cortex expressing SomArchon1 or Voltron, and zebrafish spines expressing zArchon1.

SUBMITTER: Xie ME 

PROVIDER: S-EPMC8095336 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2931774 | biostudies-literature
| S-EPMC6202643 | biostudies-literature
| S-EPMC6941988 | biostudies-literature
| S-EPMC3715760 | biostudies-other
| S-EPMC6249210 | biostudies-other
| S-EPMC6413708 | biostudies-literature
| S-EPMC4564826 | biostudies-literature
| S-EPMC4136386 | biostudies-literature
| S-EPMC7767970 | biostudies-literature
| S-EPMC2396672 | biostudies-literature