The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice.

ABSTRACT:

Background

Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. At present, vaccine immunity is still the most effective method to control the spread of PED. In this study, we have constructed a novel recombinant L. casei-OMP16-PEDVS strain expressing PEDVS protein of PEDV and OMP16 protein of Brucella abortus strain. To know the immunogenicity of the recombinant L. casei-OMP16-PEDVS candidate vaccine, it was compared with BL21-OMP16-PEDVS-F, BL21-OMP16-PEDVS, and BL21-PEDVS recombinant protein.

Results

The results showed that we could detect higher levels of IgG, neutralizing antibody, IL-4, I

SUBMITTER: Li X 

PROVIDER: S-EPMC8097780 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature