Unknown

Dataset Information

0

Chemoproteomic profiling of itaconations in Salmonella.


ABSTRACT: Itaconate is an immunoregulatory and anti-bacterial metabolite, and plays important roles in host-pathogen interactions. Chemoproteomic strategies have been used to explore the anti-inflammatory effects of itaconate on activated macrophages and it has been found that many key proteins in immune pathways were modified; however, how itaconate modulates pathogens was not fully understood. Here, we have designed and synthesized a series of itaconate-based bioorthogonal probes, which enable quantitative and site-specific profiling of itaconated proteins and sites in Salmonella. Among many proteins related to energy metabolism, we identified a key enzyme involved in the glyoxylate cycle, isocitrate lyase (ICL), as the most prominent target. Covalent modification of the active-site cysteine in ICL by itaconate abolishes the enzyme activity and suppresses bacterial growth. Our chemoproteomic study has uncovered the wide array of itaconation targets in Salmonella and provided a comprehensive resource for understanding the anti-bacterial function of this intriguing metabolite.

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8098682 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5445530 | biostudies-literature
| S-EPMC6760989 | biostudies-literature
| S-EPMC6688853 | biostudies-literature
| S-EPMC8441202 | biostudies-literature
| S-EPMC5325178 | biostudies-literature
| S-EPMC4765928 | biostudies-literature
| S-EPMC6535767 | biostudies-literature
| S-EPMC6642002 | biostudies-literature
| S-EPMC4227742 | biostudies-literature
| S-EPMC8179411 | biostudies-literature