Ontology highlight
ABSTRACT:
SUBMITTER: Hatzios SK
PROVIDER: S-EPMC4765928 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Nature chemical biology 20160222 4
Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae proteases were consistently active in infected rabbits, and one, VC0157 (renamed IvaP), was also active in human choleric stool. Inactivation of IvaP influenced the activity of other secreted V. cholerae and ...[more]