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Tissue topography steers migrating Drosophila border cells.

ABSTRACT: Moving cells can sense and respond to physical features of the microenvironment; however, in vivo, the significance of tissue topography is mostly unknown. Here, we used Drosophila border cells, an established model for in vivo cell migration, to study how chemical and physical information influences path selection. Although chemical cues were thought to be sufficient, live imaging, genetics, modeling, and simulations show that microtopography is also important. Chemoattractants promote predominantly posterior movement, whereas tissue architecture presents orthogonal information, a path of least resistance concentrated near the center of the egg chamber. E-cadherin supplies a permissive haptotactic cue. Our results provide insight into how cells integrate and prioritize topographical, adhesive, and chemoattractant cues to choose one path among many.

SUBMITTER: Dai W

PROVIDER: S-EPMC8103818 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Tissue topography steers migrating <i>Drosophila</i> border cells.

Dai Wei W Guo Xiaoran X Cao Yuansheng Y Mondo James A JA Campanale Joseph P JP Montell Brandon J BJ Burrous Haley H Streichan Sebastian S Gov Nir N Rappel Wouter-Jan WJ Montell Denise J DJ

Science (New York, N.Y.) 20201101 6519

Moving cells can sense and respond to physical features of the microenvironment; however, in vivo, the significance of tissue topography is mostly unknown. Here, we used <i>Drosophila</i> border cells, an established model for in vivo cell migration, to study how chemical and physical information influences path selection. Although chemical cues were thought to be sufficient, live imaging, genetics, modeling, and simulations show that microtopography is also important. Chemoattractants promote p ...[more]

PMID: 33214282

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