Quantitative visualization of endocytic trafficking through photoactivation of fluorescent proteins.

ABSTRACT: Endocytic trafficking controls the density of molecules at the plasma membrane and by doing so, the cell surface profile, which in turn determines how cells interact with their environment. A full apprehension of any cellular process necessitates understanding how proteins associated with the plasma membrane are endocytosed, how they are sorted after internalization, and if and how they are recycled to the plasma membrane. To date, it is still difficult to experimentally gain access to this information, even more to do it in a quantitative way. Here we present a toolset based on photoactivation of fluorescent proteins that enabled us to generate quantitative information on endocytosis, incorporation into sorting and recycling endosomes, delivery from endosomes to the plasma membrane, and o

SUBMITTER: Ecker M 

PROVIDER: S-EPMC8108533 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature