Unknown

Dataset Information

0

Regulation of gene expression in the bovine blastocyst by colony-stimulating factor 2 is disrupted by CRISPR/Cas9-mediated deletion of CSF2RA.


ABSTRACT: Colony-stimulating factor 2 (CSF2) functions in the reproductive tract to modulate the function of the preimplantation embryo. The β subunit of the CSF2 receptor (CSF2RB) is not expressed in the embryo, and signal transduction is therefore different than for myeloid cells where the receptor is composed of α (CSF2RA) and β subunits. Here, we produced embryos in which exons 5 and 6 of CSF2RA were disrupted using the CRISPR/Cas 9 system to test whether CSF2RA signaling was essential for actions of CSF2 in the bovine embryo. Wild-type and CSF2RA knockout embryos were treated with 10 ng/mL CSF2 or vehicle at day 5 of development. Blastocysts were harvested at day 8 to determine transcript abundance of 90 genes by real-time polymerase chain reaction (PCR). Responses in female blastocysts were examined separately from male blastocysts because actions of CSF2 are sex-dependent. For wild-type embryos, CSF2 altered expression of 10 genes in females and 20 in males. Only three genes were affected by CSF2 in a similar manner for both sexes. Disruption of CSF2RA prevented the effect of CSF2 on expression for 9 of 10 CSF2-regulated genes in females and 19 of 20 genes in males. The results confirm the importance of CSF2RA for regulation of gene expression by CSF2 in the blastocyst.

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC8111236 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4850677 | biostudies-literature
| S-EPMC8055050 | biostudies-literature
2021-01-14 | GSE164728 | GEO
| S-EPMC9174410 | biostudies-literature
| S-EPMC10642290 | biostudies-literature
| S-EPMC5542798 | biostudies-literature
| S-EPMC5481315 | biostudies-other
| S-EPMC4118159 | biostudies-literature
| S-EPMC6321276 | biostudies-literature
| S-EPMC8167824 | biostudies-literature