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Effects of salinity on the simultaneous anammox and denitrification process: performance, sludge morphology and shifts in microbial communities.

ABSTRACT: In this study, the long-term effects of different salinities on the performance, sludge morphology and shifts in microbial communities were studied in a simultaneous anammox and denitrification (SAD) process at a C/N ratio of 0.5. Stable nitrogen removal efficiencies of 86.96 and 84.58% and nitrogen removal rates of 0.95 and 0.93 kg (m³ d)^-1 could be achieved under low (25 mmol l^-1) and moderate (50 mmol l^-1) salinity, respectively. However, the performance collapsed when the system was exposed to high salinity (100 mmol l^-1). The content of extracellular polymeric substances increased as salinity increased, which resulted in larger sizes of granular sludge under low and moderate salinities. Nevertheless, high salinity shock disintegrated granular sludge, thereby decreasing the average granule size. The Illumina-Miseq sequencing results revealed that Candidatus Jettenia was the sole salinity-tolerant AnAOB genus during the entire operation, whereas the main denitrification bacterial genera shifted from Denitrisoma under low salinity to Denitrisoma, Thauera and Ignavibacterium under high salinity. The results of this study provide a comprehensive and practical evaluation of the SAD process for organic nitrogen-rich saline wastewater treatment.

SUBMITTER: Wang Z

PROVIDER: S-EPMC8113906 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Simultaneous nitrification-denitrification (SND) is, in theory, a key advantage of aerobic granular sludge systems over conventional activated sludge systems. But practical experience and literature suggests that SND and thus total nitrogen removal are limited during treatment of municipal wastewater using AGS systems. This study thus aims at quantifying the extent and understanding the mechanisms of SND during treatment of municipal wastewater with aerobic granular sludge (AGS) systems. Experiments (long-term and batch-tests) as well as mathematical modelling were performed. Our experimental results demonstrate that SND is significantly limited during treatment of low-strength municipal wastewater with AGS systems (14-39%), while almost full SND is observed when treating synthetic influent containing only diffusible substrate (90%). Our simulations demonstrate that the main mechanisms behind limited SND are (1) the dynamics of anoxic zone formation inside the granule, (2) the diffusibility and availability of electron-donors in those zones and (3) the aeration mode. The development of anoxic zones is driven by the utilisation of oxygen in the upper layers of the granule leading to transport limitations of oxygen inside the granule; this effect is closely linked to granule size and wastewater composition. Development of anoxic zones during the aerobic phase is limited for small granules at constant aeration at bulk dissolved oxygen (DO) concentration of 2 mgO2 L-1, and anoxic zones only develop during a brief period of the aerated phase for large granules. Modelling results further indicate that a large fraction of electron-donors are actually utilised in aerobic rather than anoxic redox zones - in the bulk or at the granule surface. Thus, full SND cannot be achieved with AGS treating low strength municipal wastewater if a constant DO is maintained during the aeration phase. Optimised aeration strategies are therefore required. 2-step and alternating aeration are tested successfully using mathematical modelling and increase TN removal to 40-79%, without compromising nitrification, and by shifting electron-donor utilisation towards anoxic redox conditions.

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Effects of salinity on the simultaneous anammox and denitrification process: performance, sludge morphology and shifts in microbial communities.

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