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Role of Hydraulic Signal and ABA in Decrease of Leaf Stomatal and Mesophyll Conductance in Soil Drought-Stressed Tomato.

ABSTRACT: Drought reduces leaf stomatal conductance (g_s) and mesophyll conductance (g_m). Both hydraulic signals and chemical signals (mainly abscisic acid, ABA) are involved in regulating g_s. However, it remains unclear what role the endogenous ABA plays in g_m under decreasing soil moisture. In this study, the responses of g_s and g_m to ABA were investigated under progressive soil drying conditions and their impacts on net photosynthesis (A_n) and intrinsic water use efficiency (WUE_i) were also analyzed. Experimental tomato plants were cultivated in pots in an environment-controlled greenhouse. Reductions of g_s and g_m induced a 68-78% decline of A_n under drought conditions. While soil water potential (Ψ_soil) was over -1.01 MPa, g_s reduced as leaf water potential (Ψ_leaf) decreased, but ABA and g_m kept unchanged, which indicating g_s was more sensitive to drought than g_m. During Ψ_soil reduction from -1.01 to -1.44 MPa, Ψ_leaf still kept decreasing, and both g_s and g_m decreased concurrently following to the sustained increases of ABA content in shoot sap. The g_m was positively correlated to g_s during a drying process. Compared to g_s or g_m, WUE_i was strongly correlated with g_m/g_s. WUE_i improved within Ψ_soil range between -0.83 and -1.15 MPa. In summary, g_s showed a higher sensitivity to drought than g_m. Under moderate and severe drought at Ψ_soil ≤ -1.01 MPa, furthermore from hydraulic signals, ABA was also involved in this co-ordination reductions of g_s and g_m and thereby regulated A_n and WUE_i.

SUBMITTER: Li S

PROVIDER: S-EPMC8118518 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Background and aimsStomatal conductance has long been considered of key interest in the study of plant adaptation to water stress. The expected increase in extreme meteorological events under a climate change scenario may compromise survival in Eucalyptus globulus plantations established in south-western Spain. We investigated to what extent changes in stomatal conductance in response to high vapour pressure deficits and water shortage are mediated by hydraulic and chemical signals in greenhouse-grown E. globulus clones.MethodsRooted cuttings were grown in pots and submitted to two watering regimes. Stomatal conductance, shoot water potential, sap pH and hydraulic conductance were measured consecutively in each plant over 4 weeks under vapour pressure deficits ranging 0·42 to 2·25?kPa. Evapotranspiration, growth in leaf area and shoot biomass were also determined.Key resultsThere was a significant effect of both clone and watering regime in stomatal conductance and leaf-specific hydraulic conductance, but not in sap pH. Sap pH decreased as water potential and stomatal conductance decreased under increasing vapour pressure deficit. There was no significant relationship between stomatal conductance and leaf-specific hydraulic conductance. Stomata closure precluded shoot water potential from falling below -1·8?MPa. The percentage loss of hydraulic conductance ranged from 40 to 85 %. The highest and lowest leaf-specific hydraulic conductances were measured in clones from the same half-sib families. Water shortage reduced growth and evapotranspiration, decreases in evapotranspiration ranging from 14 to 32 % in the five clones tested.ConclusionsChanges in sap pH seemed to be a response to changes in atmospheric conditions rather than soil water in the species. Stomata closed after a considerable amount of hydraulic conductance was lost, although intraspecific differences in leaf-specific hydraulic conductance suggest the possibility of selection for improved productivity under water-limiting conditions combined with high temperatures in the early stages of growth.

Project description:BackgroundLeaf hydraulic and economics traits are critical for balancing plant water and CO2 exchange, and their relationship has been widely studied. Leaf anatomical traits determine the efficiency of CO2 diffusion within mesophyll structure. However, it remains unclear whether leaf anatomical traits are associated with leaf hydraulic and economics traits acclimation to long-term drought.ResultsTo address this knowledge gap, eight hydraulic traits, including stomatal and venation structures, four economics traits, including leaf dry mass per area (LMA) and the ratio between palisade and spongy mesophyll thickness (PT/ST), and four anatomical traits related to CO2 diffusion were measured in tomato seedlings under the long-term drought conditions. Redundancy analysis indicated that the long-term drought decreased stomatal conductance (gs) mainly due to a synchronized reduction in hydraulic structure such as leaf hydraulic conductance (Kleaf) and major vein width. Simultaneously, stomatal aperture on the adaxial surface and minor vein density (VDminor) also contributed a lot to this reduction. The decreases in mesophyll thickness (Tmes) and chlorophyll surface area exposed to leaf intercellular air spaces (Sc/S) were primarily responsible for the decline of mesophyll conductance (gm) thereby affecting photosynthesis. Drought increased leaf density (LD) thus limited CO2 diffusion. In addition, LMA may not be important in regulating gm in tomato under drought. Principal component analysis revealed that main anatomical traits such as Tmes and Sc/S were positively correlated to Kleaf, VDminor and leaf thickness (LT), while negatively associated with PT/ST.ConclusionsThese findings indicated that leaf anatomy plays an important role in maintaining the balance between water supply and CO2 diffusion responses to drought. There was a strong coordination between leaf hydraulic, anatomical, and economical traits in tomato seedlings acclimation to long-term drought.

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Role of Hydraulic Signal and ABA in Decrease of Leaf Stomatal and Mesophyll Conductance in Soil Drought-Stressed Tomato.

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