Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect <i>in vivo</i>.

ABSTRACT: Rationale: Endoglin, also known as CD105, is a homo-dimeric membrane glycoprotein required for angiogenesis and serves as a marker for cancer vasculature. In this study, we constructed a bispecific T-cell engager (BiTE) antibody that targets human endoglin and CD3 (hEND-CD3/BiTE). We examined BiTE binding to endoglin-expressing cells and its effects on the cytolytic activity of T cells and cancer development. Methods: The in vitro effects of hEND-CD3/BiTE, including binding to target cells, T-cell activation, proliferation, and cytotoxicity, were examined in endoglin-expressing 293T cells, human umbilical vascular endothelial cells, tumor-derived endothelial cells, and CD3⁺ T cells. An in vivo xenograft tumor model was established using A549 human lun

SUBMITTER: Zhong L 

PROVIDER: S-EPMC8120215 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature