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Large-scale 3D imaging of mouse cochlea using serial block-face scanning electron microscopy.


ABSTRACT: This protocol describes how to prepare intact mouse cochleae for serial block-face scanning electron microscopy (SBEM). The detailed workflow includes cochlea fixation, en bloc staining, resin embedding, X-ray microscopy-guided trimming and SBEM data acquisition. This protocol allows large-scale, nanometer-resolution three-dimensional imaging of subcellular structures in a targeted tonotopic range of the cochlea and enables fast volumetric scan at submicron resolution using a compact X-ray microscope. For complete details on the use and execution of this protocol, please refer to Hua et al. (2021).

SUBMITTER: Lu Y 

PROVIDER: S-EPMC8121772 | biostudies-literature |

REPOSITORIES: biostudies-literature

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