Beta cell adaptation to pregnancy requires prolactin action on both beta and non-beta cells.

ABSTRACT: Pancreatic islets adapt to insulin resistance of pregnancy by up regulating β-cell mass and increasing insulin secretion. Previously, using a transgenic mouse with global, heterozygous deletion of prolactin receptor (Prlr^+/-), we found Prlr signaling is important for this adaptation. However, since Prlr is expressed in tissues outside of islets as well as within islets and prolactin signaling affects β-cell development, to understand β-cell-specific effect of prolactin signaling in pregnancy, we generated a transgenic mouse with an inducible conditional deletion of Prlr from β-cells. Here, we found that β-cell-specific Prlr reduction in adult mice led to elevated blood glucose, lowed β-cell mass and blunted in vivo glucose-stimulated insulin secretion during pregnancy. When we compared gene expression profile of islets from transgenic mice with global (Prlr^+/-) versus β-cell-specific Prlr reduction (βPrlR^+/-), we found 95 differentially expressed gene, most of them down regulated in the Prlr^+/- mice in comparison to the βPrlR^+/- mice, and many of these genes regulate apoptosis, synaptic vesicle function and neuronal development. Importantly, we found that islets from pregnant Prlr^+/- mice are more vulnerable to glucolipotoxicity-induced apoptosis than islets from pregnant βPrlR^+/- mice. These observations suggest that down regulation of prolactin action during pregnancy in non-β-cells secondarily and negatively affect β-cell gene expression, and increased β-cell susceptibility to external insults.

SUBMITTER: Shrivastava V 

PROVIDER: S-EPMC8121891 | biostudies-literature |

REPOSITORIES: biostudies-literature