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Synthesis and Characterization of a Mg²⁺-Selective Probe Based on Benzoyl Hydrazine Derivative and Its Application in Cell Imaging.

ABSTRACT: A simple benzoyl hydrazine derivative P was successfully synthesized and characterized as Mg²⁺-selective fluorescent probe. The binding of P with Mg²⁺ caused an obvious fluorescence enhancement at 482 nm. The fluorescent, UV-vis spectra, ¹H-NMR, and IR spectra confirmed the formation of P-Mg²⁺ complex, and the formation of a 1:1 stoichiometry complex was proved by Job's plot and mass spectrometry. The recognition mechanism of P to Mg²⁺ was owing to the photoinduced electron transfer effect (PET). The fluorescent response was linear in the range of 0.9-4.0 µM with the detection limit of 0.3 µM Mg²⁺ in water-ethanol solution (1:9, v:v, pH10.0, 20 mM HEPES). In addition, the results of cell imaging of Mg²⁺ in Hl-7701 cells was satisfying.

SUBMITTER: Yu C

PROVIDER: S-EPMC8122791 | biostudies-literature |

REPOSITORIES: biostudies-literature

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