Neuron Replating, a Powerful and Versatile Approach to Study Early Aspects of Neuron Differentiation.

ABSTRACT: Neuron differentiation includes formation and outgrowth of neurites that differentiate into axons or dendrites. Directed neurite outgrowth is controlled by growth cones that protrude and retract actin-rich structures to sense environmental cues. These cues control local actin filament dynamics, steer growth cones toward attractants and away from repellents, and navigate neurites through the developing brain. Rodent hippocampal neurons are widely used to study the mechanisms underlying neuron differentiation. Genetic manipulation of isolated neurons including gene inactivation or reporter gene expression can be achieved by classical transfections methods, but these methods are restricted to neurons cultured for several days, after neurite formation or outgrowth. Instead, electroporation all

SUBMITTER: Schneider F 

PROVIDER: S-EPMC8143016 | biostudies-literature | 2021 May-Jun

REPOSITORIES: biostudies-literature