CRISPR Interference Efficiently Silences Latent and Lytic Viral Genes in Kaposi's Sarcoma-Associated Herpesvirus-Infected Cells.
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ABSTRACT:
SUBMITTER: Brackett K
PROVIDER: S-EPMC8146339 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
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CRISPR Interference Efficiently Silences Latent and Lytic Viral Genes in Kaposi's Sarcoma-Associated Herpesvirus-Infected Cells.
Brackett Kevin K Mungale Ameera A Lopez-Isidro Mary M Proctor Duncan A DA Najarro Guillermo G Arias Carolina C
Viruses 20210428 5
Uncovering viral gene functions requires the modulation of gene expression through overexpression or loss-of-function. CRISPR interference (CRISPRi), a modification of the CRISPR-Cas9 gene editing technology, allows specific and efficient transcriptional silencing without genetic ablation. CRISPRi has been used to silence eukaryotic and prokaryotic genes at the single-gene and genome-wide levels. Here, we report the use of CRISPRi to silence latent and lytic viral genes, with an efficiency of ~8 ...[more]
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