Unknown

Dataset Information

0

Fast Detection of SARS-CoV-2 RNA Directly from Respiratory Samples Using a Loop-Mediated Isothermal Amplification (LAMP) Test.


ABSTRACT: The availability of simple SARS-CoV-2 detection methods is crucial to contain the COVID-19 pandemic. This study examined whether a commercial LAMP assay can reliably detect SARS-CoV-2 genomes directly in respiratory samples without having to extract nucleic acids (NA) beforehand. Nasopharyngeal swabs (NPS, n = 220) were tested by real-time reverse transcription (RT)-PCR and with the LAMP assay. For RT-PCR, NA were investigated. For LAMP, NA from 26 NPS in viral transport medium (VTM) were tested. The other 194 NPS were analyzed directly without prior NA extraction (140 samples in VTM; 54 dry swab samples stirred in phosphate buffered saline). Ten NPS were tested directly by LAMP using a sous-vide cooking unit. The isothermal assay demonstrated excellent specificity (100%) but moderate sensitivity (68.8%), with a positive predictive value of 1 and a negative predictive value of 0.65 for direct testing of NPS in VTM. The use of dry swabs, even without NA extraction, improved the analytical sensitivity; up to 6% of samples showed signs of inhibition. LAMP could be performed successfully with a sous-vide cooking unit. This technique is very fast, requires little laboratory resources, and can replace rapid antigen tests or verify reactive rapid tests on-site.

SUBMITTER: Anastasiou OE 

PROVIDER: S-EPMC8146783 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC9387413 | biostudies-literature
| S-EPMC4417551 | biostudies-literature
| S-EPMC10470312 | biostudies-literature
| S-EPMC8560768 | biostudies-literature
| S-EPMC5220095 | biostudies-literature
| S-EPMC4626017 | biostudies-literature
| S-EPMC6717426 | biostudies-literature
| S-EPMC6435178 | biostudies-literature
| S-EPMC4033683 | biostudies-literature
| S-EPMC10928092 | biostudies-literature