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The Profound Influence of Lipid Composition on the Catalysis of the Drug Target NADH Type II Oxidoreductase.


ABSTRACT: Lipids play a pivotal role in cellular respiration, providing the natural environment in which an oxidoreductase interacts with the quinone pool. To date, it is generally accepted that negatively charged lipids play a major role in the activity of quinone oxidoreductases. By changing lipid compositions when assaying a type II NADH:quinone oxidoreductase, we demonstrate that phosphatidylethanolamine has an essential role in substrate binding and catalysis. We also reveal the importance of acyl chain composition, specifically c14:0, on membrane-bound quinone-mediated catalysis. This demonstrates that oxidoreductase lipid specificity is more diverse than originally thought and that the lipid environment plays an important role in the physiological catalysis of membrane-bound oxidoreductases.

SUBMITTER: Godoy-Hernandez A 

PROVIDER: S-EPMC8156991 | biostudies-literature |

REPOSITORIES: biostudies-literature

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