Effect of Serum on Electrochemical Detection of Bioassays Having Ag Nanoparticle Labels.

ABSTRACT: The effect of serum on electrochemical detection of bioassays having silver nanoparticle (AgNP) detection labels was investigated. Both a model assay and an antigen-specific sandwich bioassay for the heart failure marker NT-proBNP were examined. In both cases, the AgNP labels were conjugated to a detection antibody. Electrochemical detection was carried out using a galvanic exchange/anodic stripping voltammetry method in which Au³⁺ exchanges with AgNP labels. The assays were carried out using a paper-based electrode platform. The bioassays were exposed to different serum conditions prior to and during detection. There are three important outcomes reported in this article. First, both the model- and antigen-specific assays could be formed in undiluted serum with no detectable int

SUBMITTER: Pollok NE 

PROVIDER: S-EPMC8164997 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature