Focused peptide library screening as a route to a superior affinity ligand for antibody purification.

ABSTRACT: Affinity chromatography is the linchpin of antibody downstream processing and typically relies on bacterial immunoglobulin (Ig)-binding proteins, epitomized by staphylococcal protein A-based ligands. However, such affinity ligands are fairly costly and suffer from chemical instability, leading to ligand denaturation and leaching from chromatographic support. Innovations in this area are aimed at developing robust and highly selective antibody ligands capable of withstanding harsh column sanitization conditions. We report the development and first-stage characterization of a selective short linear peptide ligand of the IgG Fc region capable of capturing all four IgG subclasses. The ligand was discovered through in vitro directed evolution. A focused phage-display library based on a previous

SUBMITTER: Bozovicar K 

PROVIDER: S-EPMC8173005 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature