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Naive stem cell blastocyst model captures human embryo lineage segregation.

ABSTRACT: Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.

SUBMITTER: Yanagida A

PROVIDER: S-EPMC8189436 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Naive stem cell blastocyst model captures human embryo lineage segregation.

Yanagida Ayaka A Spindlow Daniel D Nichols Jennifer J Dattani Anish A Smith Austin A Guo Ge G

Cell stem cell 20210505 6

Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high ...[more]

PMID: 33957081

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