Ontology highlight
ABSTRACT:
SUBMITTER: Song K
PROVIDER: S-EPMC8195388 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Song Kai K Sun Jingjing J Wang Wei W Hao Jianhua J
Frontiers in microbiology 20210528
In this study, the cgt gene <i>my</i>20, which encodes cyclodextrin glycosyltransferase (CGTase) and was obtained by the metagenome sequencing of marine microorganisms from the Mariana Trench, was codon optimized and connected to pET-24a for heterologous expression in <i>Escherichia coli</i> BL21(DE3). Through shaking flask fermentation, the optimized condition for recombinant CGTase expression was identified as 20°C for 18 h with 0.4 mM of isopropyl β-D-L-thiogalactopyranoside. The recombinant ...[more]