ABSTRACT: Increasing heavy metal pollution in the environment and the fact that pine needles are good bio-monitors for air pollutants was the reason to investigate their accumulation in pine needles in Vienna (Austria). Pinus nigra is widespread over the city, thus allowing the study of different parameters influencing metal accumulation. The sampling sites were chosen based on traffic volume (low, medium, high). Fresh shoots were collected alongside one-year-old needles once per week from May to August 2015. The needle samples were washed and dried prior to acidic microwave-assisted digestion followed by quantitative determination using spectrometric methods. The investigation was focused on the following elements: Ag, Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, Pb, Se, Sr, U, V, and Zn. The one-year-old needles mainly contained higher contents of elements than fresh shoots; in many cases, the values differed statistically significantly. By correlating needle elemental contents with the sampling site, statistically significant differences were registered for the majority of the investigated elements. These differences originate from the local traffic situation, soil elemental levels, translocation processes, and not traffic-related sources. No general trend of metal accumulation from spring to summer was registered.
