Visualization of β-adrenergic receptor dynamics and differential localization in cardiomyocytes.

ABSTRACT: A key question in receptor signaling is how specificity is realized, particularly when different receptors trigger the same biochemical pathway(s). A notable case is the two β-adrenergic receptor (β-AR) subtypes, β₁ and β₂, in cardiomyocytes. They are both coupled to stimulatory G_s proteins, mediate an increase in cyclic adenosine monophosphate (cAMP), and stimulate cardiac contractility; however, other effects, such as changes in gene transcription leading to cardiac hypertrophy, are prominent only for β₁-AR but not for β₂-AR. Here, we employ highly sensitive fluorescence spectroscopy approaches, in combination with a fluorescent β-AR antagonist, to determine the presence and dynamics of the endogenous receptors on the outer plasma me

SUBMITTER: Bathe-Peters M 

PROVIDER: S-EPMC8201832 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature