Unknown

Dataset Information

0

Detection of Vibrio vulnificus in Seafood With a DNAzyme-Based Biosensor.


ABSTRACT: Vibrio vulnificus is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the in vitro detection of V. vulnificus. After screening and mutation, a DNAzyme that we denominated "RFD-VV-M2" exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 103 CFU/ml, and results could be obtained within 5-10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with V. vulnificus. Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for V. vulnificus in aquatic products.

SUBMITTER: Fan S 

PROVIDER: S-EPMC8213197 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7677453 | biostudies-literature
| S-EPMC7409051 | biostudies-literature
| S-EPMC8615721 | biostudies-literature
| S-EPMC8659037 | biostudies-literature
| S-EPMC4091370 | biostudies-literature
| S-EPMC3360785 | biostudies-literature
2008-08-07 | GSE10633 | GEO
| S-EPMC4655907 | biostudies-other
2018-11-23 | PXD009215 | Pride
| S-EPMC6630308 | biostudies-literature