Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity.

ABSTRACT:

Objective

This study aimed to investigate the regulatory effects of repressor element-1 silencing transcription factor (REST) on the glutamate receptors and immediate early genes (IEGs) in the SH-SY5Y cells.

Methods

The genes regulated by REST were screened by bioinformatics between AD patients and the control group. Then, SH-SY5Y cells were treated with 10 μM Aβ or REST siRNA/cDNA, and the expressions of synaptic genes and IEGs were detected. Moreover, the protein expression of synaptophysin and PSD-95 was detected by Western blotting in the primary mouse hippocampal neurons.

Results

Firstly, 464 differentially expressed genes regulated by REST were identified between Alzheimer's disease (AD) patients and controls, and REST was closely related to the glutamatergic sy

SUBMITTER: Xu C 

PROVIDER: S-EPMC8221361 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature