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Structural basis of GABA_B receptor-G_i protein coupling.

ABSTRACT: G-protein-coupled receptors (GPCRs) have central roles in intercellular communication^1,2. Structural studies have revealed how GPCRs can activate G proteins. However, whether this mechanism is conserved among all classes of GPCR remains unknown. Here we report the structure of the class-C heterodimeric GABA_B receptor, which is activated by the inhibitory transmitter GABA, in its active form complexed with G_i1 protein. We found that a single G protein interacts with the GB2 subunit of the GABA_B receptor at a site that mainly involves intracellular loop 2 on the side of the transmembrane domain. This is in contrast to the G protein binding in a central cavity, as has been observed with other classes of GPCR. This binding mode results from the active form of the transmembrane domain of this GABA_B receptor being different from that of other GPCRs, as it shows no outside movement of transmembrane helix 6. Our work also provides details of the inter- and intra-subunit changes that link agonist binding to G-protein activation in this heterodimeric complex.

SUBMITTER: Shen C

PROVIDER: S-EPMC8222003 | biostudies-literature |

REPOSITORIES: biostudies-literature

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