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Methods for in vitro CRISPR/CasRx-Mediated RNA Editing.

ABSTRACT: Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the in vitro methods of utilizing this powerful RNA editing platform and determine the RNA editing efficiencies for CasRx with different forms of guide RNAs (also known as gRNA or sgRNA).

SUBMITTER: Chuang YF

PROVIDER: S-EPMC8226256 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Methods for <i>in vitro</i> CRISPR/CasRx-Mediated RNA Editing.

Chuang Yu-Fan YF Wang Peng-Yuan PY Kumar Satheesh S Lama Suraj S Lin Fan-Li FL Liu Guei-Sheung GS

Frontiers in cell and developmental biology 20210611

Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the <i>in vitro</i> methods of utilizing this powerful RNA editing ...[more]

PMID: 34178991

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