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Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase.

ABSTRACT: Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the AI-seq (artificial incorporation modified nucleobase for sequencing), a "base substitution", which not only is capable of profiling uracil at single-nucleotide resolution and showing its centromeric enrichment but could also reveal that the identified uracil sites are derived from cytosine deamination. All the results indicate the potential biological significance of uracil as the epigenetic modification.

SUBMITTER: Wang Y

PROVIDER: S-EPMC8227591 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase.

Wang Yafen Y Zhang Xiong X Han Shaoqing S Yang Wei W Chen Zonggui Z Wu Fan F Liu Jizhou J Weng Xiaocheng X Zhou Xiang X

ACS central science 20210428 6

Deamination of cytosine and dUMP misincorporation have been found to be capable of producing uracil in the genome. This study presents the AI-seq (artificial incorporation modified nucleobase for sequencing), a "base substitution", which not only is capable of profiling uracil at single-nucleotide resolution and showing its centromeric enrichment but could also reveal that the identified uracil sites are derived from cytosine deamination. All the results indicate the potential biological signifi ...[more]

PMID: 34235258

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