Genes Relevant to Tissue Response to Cancer Therapy Display Diurnal Variation in mRNA Expression in Human Oral Mucosa.

ABSTRACT:

Background

To address a critical gap for application of cancer chronotherapy of when would be the best time(s) for treating an individual cancer patient, we conducted a pilot study to characterize diurnal variations of gene expression in oral mucosal tissue, which is vulnerable to damage from cancer therapies.

Methods

We conducted RNA-seq assay on individual oral mucosal samples collected from 11 healthy volunteers every 4 hours (6 time points). Using a cosine-based method, we estimated the individual and average values of peak-time and amplitude for each gene. Correlations between gene expression peak-times and age was examined, adjusting for individual's sleep timing.

Results

Among candidate gene pathways that are relevant to treatment response, 7 of 16 genes (PE

SUBMITTER: Gu F 

PROVIDER: S-EPMC8231453 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature