Dataset Information

Time to blastulation is superior to individual components of embryo grading for live-birth prediction.

ABSTRACT:

Objective

To compare components of the embryo grading system with time for blastocyst formation (i.e., day of embryo transfer) for predicting live-birth rate in frozen embryo transfer cycles.

Design

Retrospective cohort study.

Setting

University-affiliated fertility clinic.

Patients

From January 2015 to October 2018, 870 frozen embryos transferred in a total of 509 women and 728 cycles at our institution.

Interventions

None.

Main outcome measures

Probability of live birth per cycle.

Results

In unadjusted analysis of embryo grading components, both inner cell mass (ICM) and trophectoderm grades demonstrated a correlation with live-birth rates. However, this effect was lost in the ICM subgroup analysis by day of embryo transfer and preserved only in declining trophectoderm grades of day-6 transfers. In the adjusted analysis for prediction of live birth, only day of transfer was statistically significant. When assessing the composite score by Society for Assisted Reproductive Technology (SART) embryo grading, good embryos that blastulated on day 6 were statistically significantly less likely than day-5 embryos to result in live birth (risk ratio 0.70; 95% confidence interval, 0.58-0.85). Finally, in a predictive model adjusted for all individual components of embryo grade, the day of blastulation was the only statistically significant contributor.

Conclusions

Time to blastulation is superior to other individual components of embryonic grading for prediction of live birth.

SUBMITTER: Moustafa SM

PROVIDER: S-EPMC8244320 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Time to blastulation is superior to individual components of embryo grading for live-birth prediction.

Moustafa Sarah M SM Rosen Emma M EM Boylan Caitlin C Mersereau Jennifer E JE

F&S reports 20201008 3

<h4>Objective</h4>To compare components of the embryo grading system with time for blastocyst formation (i.e., day of embryo transfer) for predicting live-birth rate in frozen embryo transfer cycles.<h4>Design</h4>Retrospective cohort study.<h4>Setting</h4>University-affiliated fertility clinic.<h4>Patients</h4>From January 2015 to October 2018, 870 frozen embryos transferred in a total of 509 women and 728 cycles at our institution.<h4>Interventions</h4>None.<h4>Main outcome measures</h4>Probab ...[more]

PMID: 34223251

Similar Datasets

Project description:Study questionCan time-lapse imaging systems make it possible to identify novel early non-invasive biomarkers to predict live birth?Summary answerFrom mostly high-grade embryos, out of 35 morphometric, morphologic and morphokinetic variables, only pronuclei (PN) position at time of PN juxtaposition and the absence of multinucleated blastomeres at the 2-cell stage (MNB2cell), were potentially associated with live birth.What is known alreadyPrevious studies indicate that some kinetic markers may be predictive of blastocyst development and embryonic implantation. Certain teams have suggested including some of them in decisional algorithms for embryo transfers.Study design, size, durationUsing a time-lapse incubator (EmbryoScope, Unisense FertiliTech), we retrospectively explored the associations between the morphometric, morphologic and morphokinetic parameters of oocytes, zygotes and embryos, and their associations with live birth. This study assessed 232 embryos from single embryo transfers after ICSI cycles performed between January 2014 and December 2017.Participants/materials, setting, methodsThe morphometric, morphologic and morphokinetic parameters (18, 4 and 13, respectively) of oocytes, zygotes and early embryos were studied retrospectively. The associations between these parameters were examined using a Spearman's correlation, Mann-Whitney or chi-squared test as appropriate. We examined whether these parameters were associated with outcomes in univariate and multivariate logistic regression analyses.Main results and the role of chanceCentral PN juxtaposition was associated with a 2-fold increase in the odds of live birth (OR = 2.20; 95% CI, [1.26-3.89]; P = 0.006), while the presence of MNB2cell was associated with half the odds of live birth (OR = 0.51; 95% CI, [0.27-0.95]; P = 0.035). These two parameters were independent of embryo kinetics. The 33 remaining parameters had no significant association with the capacity of transferred embryos to develop to term.Limitations, reasons for cautionEven though the population size was relatively small, our analyses were based on homogeneous cycles, i.e. young women whose transferred embryos were found to be high-grade according to conventional morphology evaluation. In addition, our conclusions were established from a specific, highly selected population, so other study populations, such as women in an older age bracket, may yield different results. Finally, because we assessed day 2/3 transfers, our findings cannot be generalized to embryos cultured up to the blastocyst stage.Wider implications of the findingsIt would be interesting to explore, prospectively, whether PN localisation is a relevant measure to predict embryo development when added into further algorithms and whether this parameter could be suitable for use in other IVF clinics. Further studies are needed, notably to explore the added value of timing evaluation in cohorts of embryos with low or intermediate morphology grade, as well as in other maternal populations (i.e. older women).Study funding/competing interest(s)No external funding was used for this study. P. Sagot received funding from the following commercial companies: Merck Serono, Finox Biotech, Ferring, MSD France SAS, Teva Sante ́ SAS, Allergan France, Gedeon Richter France, Effik S.A., Karl Storz Endoscopie France, GE Medical Systems SCS, Laboratoires Genevrier, H.A.C. Pharma and Ipsen.All the authors confirm that none of this funding was used to support the research in this study. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the journal policies on sharing data and materials.

Project description:Previous research suggests statistical associations between season of birth (SOB) with prevalence of neurobehavioral disorders such as schizophrenia and bipolar disorder, personality traits such as novelty and sensation seeking, and suicidal behavior. These effects are thought to be mediated by seasonal differences in perinatal photoperiod, which was recently shown to imprint circadian clock neurons and behavior in rodents. However, it is unknown whether SOB is associated with any measurable differences in the normal human adult brain, and whether individual SOB can be deduced based on phenotype. Here I show that SOB predicts morphological differences in brain structure, and that MRI scans carry spatially distributed information allowing significantly above chance prediction of an individual's SOB. Using an open source database of over 550 structural brain scans, Voxel-Based Morphometry (VBM) analysis showed a significant SOB effect in the left superior temporal gyrus (STG) in males (p=0.009, FWE whole-brain corrected), with greater gray matter volumes in fall and winter births. A cosinor analysis revealed a significant annual periodicity in the left STG gray matter volume (Zero Amplitude Test: p<5×10(-7)), with a peak towards the end of December and a nadir towards the end of June, suggesting that perinatal photoperiod accounts for this SOB effect. Whole-brain VBM maps were used as input features to multivariate machine-learning based analyses to classify SOB. Significantly greater than chance prediction was achieved in females (overall accuracy 35%, p<0.001), but not in males (overall accuracy 26%, p=0.45). Pairwise binary classification in females revealed that the highest discrimination was obtained for winter vs. summer classification (peak area under the ROC curve=0.71, p<0.0005). Discriminating regions included fusiform and middle temporal gyrus, inferior and superior parietal lobe, cerebellum, and dorsolateral and dorsomedial prefrontal cortex. Results indicate that SOB is detectable with MRI, imply that SOB exerts effects on the developing human brain that persist through adulthood, and suggest that neuroimaging may be a valuable intermediate phenotype in bridging the gap between SOB and personality and neurobehavioral disorders.

Dataset Information

Time to blastulation is superior to individual components of embryo grading for live-birth prediction.

Objective

Design

Setting

Patients

Interventions

Main outcome measures

Results

Conclusions

Publications

Time to blastulation is superior to individual components of embryo grading for live-birth prediction.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets