Unknown

Dataset Information

0

Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging.


ABSTRACT: Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.

SUBMITTER: Kim DH 

PROVIDER: S-EPMC8246296 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3007670 | biostudies-literature
| S-EPMC3353744 | biostudies-literature
| S-EPMC7028321 | biostudies-literature
| S-EPMC2820094 | biostudies-literature
2013-03-14 | GSE45146 | GEO
2013-06-28 | GSE34137 | GEO
| S-EPMC3400377 | biostudies-literature
| S-EPMC6104530 | biostudies-literature
| S-EPMC8605398 | biostudies-literature
| S-EPMC8544604 | biostudies-literature