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Crassulacean acid metabolism species differ in the contribution of C₃ and C₄ carboxylation to end of day CO₂ fixation.

ABSTRACT: Crassulacean acid metabolism (CAM) is a photosynthetic pathway that temporally separates the nocturnal CO₂ uptake, via phosphoenolpyruvate carboxylase (PEPC, C₄ carboxylation), from the diurnal refixation by Rubisco (C₃ carboxylation). At the end of the day (CAM-Phase IV), when nocturnally stored CO₂ has depleted, stomata reopen and allow additional CO₂ uptake, which can be fixed by Rubisco or by PEPC. This work examined the CO₂ uptake via C₃ and C₄ carboxylation in phase IV in the CAM species Phalaenopsis "Sacramento" and Kalanchoe blossfeldiana "Saja." Short blackout periods during phase IV caused a sharp drop in CO₂ uptake in K. blossfeldiana but not in Phalaenopsis, indicating strong Rubisco activity only in K. blossfeldiana. Chlorophyll fluorescence revealed a progressive decrease in ΦPSII in Phalaenopsis, implying decreasing Rubisco activity, while ΦPSII remained constant in phase IV in K. blossfeldiana. However, short switching to 2% O₂ indicated the presence of photorespiration and thus Rubisco activity in both species throughout phase IV. Lastly, in Phalaenopsis, accumulation of starch in phase IV occurred. These results indicate that in Phalaenopsis, PEPC was the main carboxylase in phase IV, although Rubisco remained active throughout the whole phase. This will lead to double carboxylation (futile cycling) but may help to avoid photoinhibition.

SUBMITTER: van Tongerlo E

PROVIDER: S-EPMC8246577 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Background and aimsPlants with crassulacean acid metabolism (CAM) can be divided into two groups according to the major carbohydrates used for malic acid synthesis, either polysaccharide (starch) or monosaccharide (hexose). This is related to the mechanism and affects energy metabolism in the two groups. In Kalanchoë pinnata and K. daigremontiana, which utilize starch, ATP-dependent phosphofructokinase (tonoplast inorganic pyrophosphatase) activity is greater than inorganic pyrophosphate-dependent phosphofructokinase (tonoplast adenosine triphosphatase) activity, but the reverse is the case in pineapple (Ananas comosus) utilizing hexose. To test the hypothesis that the energy metabolism of the two groups differs, day-night changes in the contents of ATP, ADP, AMP, inorganic phosphate (Pi), phosphoenolpyruvate (PEP) and inorganic pyrophosphate (PPi) in K. pinnata and K. daigremontiana leaves and in pineapple chlorenchyma were analysed.MethodsThe contents of energy-rich compounds were measured spectrophotometrically in extracts of tissue sampled in the light and dark, using potted plants, kept for 15 d before the experiments in a growth chamber.Key resultsIn the three species, ATP content and adenylate energy charge (AEC) increased in the dark and decreased in the light, in contrast to ADP and AMP. Changes in ATP and AEC were greater in Kalanchoë leaves than in pineapple chlorenchyma. PPi content in the three species increased in the dark, but on illumination it decreased rapidly and substantially, remaining little changed through the rest of the light period. Pi content of Kalanchoë leaves did not change between dark and light, whereas Pi in pineapple chlorenchyma increased in the dark and decreased in the light, and the changes were far greater than in Kalanchoë leaves. Light-dark changes in PEP content in the three species were similar.ConclusionsThese results corroborate our hypothesis that day-night changes in the contents of energy-rich compounds differ between CAM species and are related to the carbohydrate used for malic acid synthesis.

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Crassulacean acid metabolism species differ in the contribution of C₃ and C₄ carboxylation to end of day CO₂ fixation.

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Crassulacean acid metabolism species differ in the contribution of C₃ and C₄ carboxylation to end of day CO₂ fixation.