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Automated Glycan Assembly of ¹⁹ F-labeled Glycan Probes Enables High-Throughput NMR Studies of Protein-Glycan Interactions.

ABSTRACT: Protein-glycan interactions mediate important biological processes, including pathogen host invasion and cellular communication. Herein, we showcase an expedite approach that integrates automated glycan assembly (AGA) of ¹⁹ F-labeled probes and high-throughput NMR methods, enabling the study of protein-glycan interactions. Synthetic Lewis type 2 antigens were screened against seven glycan binding proteins (GBPs), including DC-SIGN and BambL, respectively involved in HIV-1 and lung infections in immunocompromised patients, confirming the preference for fucosylated glycans (Le^x , H type 2, Le^y ). Previously unknown glycan-lectin weak interactions were detected, and thermodynamic data were obtained. Enzymatic reactions were monitored in real-time, delivering kinetic parameters. These results demonstrate the utility of AGA combined with ¹⁹ F NMR for the discovery and characterization of glycan-protein interactions, opening up new perspectives for ¹⁹ F-labeled complex glycans.

SUBMITTER: Fittolani G

PROVIDER: S-EPMC8252726 | biostudies-literature |

REPOSITORIES: biostudies-literature

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