Kdm3b haploinsufficiency impairs the consolidation of cerebellum-dependent motor memory in mice.

ABSTRACT: Histone modifications are a key mechanism underlying the epigenetic regulation of gene expression, which is critically involved in the consolidation of multiple forms of memory. However, the roles of histone modifications in cerebellum-dependent motor learning and memory are not well understood. To test whether changes in histone methylation are involved in cerebellar learning, we used heterozygous Kdm3b knockout (Kdm3b^+/-) mice, which show reduced lysine 9 on histone 3 (H3K9) demethylase activity. H3K9 di-methylation is significantly increased selectively in the granule cell layer of the cerebellum of Kdm3b^+/- mice. In the cerebellum-dependent optokinetic response (OKR) learning, Kdm3b^+/- mice show deficits in memory consolidation, whereas they are normal in basal oculomotor performance and OKR acquisition. In addition, RNA-seq analyses revealed that the expression levels of several plasticity-related genes were altered in the mutant cerebellum. Our study suggests that active regulation of histone methylation is critical for the consolidation of cerebellar motor memory.

SUBMITTER: Kim YG 

PROVIDER: S-EPMC8254933 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature