Ontology highlight
ABSTRACT:
SUBMITTER: Li Z
PROVIDER: S-EPMC8256697 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Li Zhuang Z Zhang Heng H Xiao Renjian R Han Ruijie R Chang Leifu L
Nature chemical biology 20210125 4
Cas12g, the type V-G CRISPR-Cas effector, is an RNA-guided ribonuclease that targets single-stranded RNA substrate. The CRISPR-Cas12g system offers a potential platform for transcriptome engineering and diagnostic applications. We determined the structures of Cas12g-guide RNA complexes in the absence and presence of target RNA by cryo-EM to a resolution of 3.1 Å and 4.8 Å, respectively. Cas12g adopts a bilobed structure with miniature REC2 and Nuc domains, whereas the guide RNAs fold into a flip ...[more]