Ontology highlight
ABSTRACT: Background
Autoreactive B cells are well recognized as key participants in the pathogenesis of systemic lupus erythematosus (SLE). However, elucidating the particular subset of B cells in producing anti-dsDNA antibodies is limited due to their B cell heterogeneity. This study aimed to identify peripheral B cell subpopulations that display autoreactivity to DNA and contribute to lupus pathogenesis.Methods
Flow cytometry was used to detect total B cell subsets (n = 20) and DNA autoreactive B cells (n = 15) in SLE patients' peripheral blood. Clinical disease activities were assessed in SLE patients using modified SLEDAI-2 K and used for correlation analyses with expanded B cell subsets and DNA autoreactive B cells.Results
The increases of circulating double negative 2 (DN2) and activated naïve (aNAV) B cells were significantly observed in SLE patients. Expanded B cell subsets and DNA autoreactive B cells represented a high proportion of aNAV B cells with overexpression of CD69 and CD86. The frequencies of aNAV B cells in total B cell populations were significantly correlated with modified SLEDAI-2 K scores. Further analysis showed that expansion of aNAV DNA autoreactive B cells was more related to disease activity and serum anti-dsDNA antibody levels than to total aNAV B cells.Conclusion
Our study demonstrated an expansion of aNAV B cells in SLE patients. The association between the frequency of aNAV B cells and disease activity patients suggested that these expanded B cells may play a role in SLE pathogenesis.
SUBMITTER: Wangriatisak K
PROVIDER: S-EPMC8259008 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Wangriatisak Kittikorn K Thanadetsuntorn Chokchai C Krittayapoositpot Thamonwan T Leepiyasakulchai Chaniya C Suangtamai Thanitta T Ngamjanyaporn Pintip P Khowawisetsut Ladawan L Khaenam Prasong P Setthaudom Chavachol C Pisitkun Prapaporn P Chootong Patchanee P
Arthritis research & therapy 20210706 1
<h4>Background</h4>Autoreactive B cells are well recognized as key participants in the pathogenesis of systemic lupus erythematosus (SLE). However, elucidating the particular subset of B cells in producing anti-dsDNA antibodies is limited due to their B cell heterogeneity. This study aimed to identify peripheral B cell subpopulations that display autoreactivity to DNA and contribute to lupus pathogenesis.<h4>Methods</h4>Flow cytometry was used to detect total B cell subsets (n = 20) and DNA auto ...[more]