Unknown

Dataset Information

0

Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53.


ABSTRACT: During HIV-1 particle formation, the requisite plasma membrane curvature is thought to be solely driven by the retroviral Gag protein. Here, we reveal that the cellular I-BAR protein IRSp53 is required for the progression of HIV-1 membrane curvature to complete particle assembly. siRNA-mediated knockdown of IRSp53 gene expression induces a decrease in viral particle production and a viral bud arrest at half completion. Single-molecule localization microscopy at the cell plasma membrane shows a preferential localization of IRSp53 around HIV-1 Gag assembly sites. In addition, we observe the presence of IRSp53 in purified HIV-1 particles. Finally, HIV-1 Gag protein preferentially localizes to curved membranes induced by IRSp53 I-BAR domain on giant unilamellar vesicles. Overall, our data reveal a strong interplay between IRSp53 I-BAR and Gag at membranes during virus assembly. This highlights IRSp53 as a crucial host factor in HIV-1 membrane curvature and its requirement for full HIV-1 particle assembly.

SUBMITTER: Inamdar K 

PROVIDER: S-EPMC8260224 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4634128 | biostudies-literature
| S-EPMC3020119 | biostudies-literature
| S-EPMC3248516 | biostudies-literature
| S-EPMC7583631 | biostudies-literature
| S-EPMC4455092 | biostudies-literature
| S-EPMC4505496 | biostudies-literature
| S-EPMC2267228 | biostudies-other
| S-EPMC5640829 | biostudies-literature
| S-EPMC7044464 | biostudies-literature
| S-EPMC7255505 | biostudies-literature