The Sonic Hedgehog signaling pathway regulates autophagy and migration in ovarian cancer.

ABSTRACT:

Background

The Sonic Hedgehog (SHH) signaling pathway plays an important role in various types of human cancers including ovarian cancer; however, its function and underlying mechanism in ovarian cancer are still not entirely understood.

Methods

We detected the expressions of SHH and SQSTM1 in borderline ovarian tumor tissues, epithelial ovarian cancer (EOC) tissues and benign ovarian tumor tissues. Cyclopamine (Cyp, a well-known inhibitor of SHH signaling pathway) and chloroquine (CQ, the pharmaceutical inhibitor of autophagy) were used in vivo and in vitro (autophagic flux, CCK-8 assay, wound healing assay, transwell assay, tumor xenograft model). The mechanism of action was explored through Quantitative RT-PCR and Western Blot.

Results

We found up-regulation of SHH

SUBMITTER: Pan Y 

PROVIDER: S-EPMC8267163 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature