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Statistical evaluation of main extraction parameters in twenty plant extracts for obtaining their optimum total phenolic content and its relation to antioxidant and antibacterial activities.

ABSTRACT: The main extraction parameters were statistically evaluated for 20 medicinal plants to obtain the optimum conditions for maximum extraction of total phenolic content (TPC) in each plant. Among various extraction parameters, pH, temperature, and concentration at different levels were studied. The results were analyzed using the analysis of variance to achieve the optimum conditions of phenolic extraction for all plants. Also, investigation of the optimum antioxidant (AnOX) activities using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and antibacterial potential against common pathogenic bacteria of Staphylococcus aureus, Escherichia coli, Pseudomonas spp., and Candida spp. through disk diffusion method for the extract of all plants under the optimum total phenolic concentration of each plant extract confirmed a direct relation among bioactivity and TPC.

SUBMITTER: Kadir DH

PROVIDER: S-EPMC8269642 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Statistical evaluation of main extraction parameters in twenty plant extracts for obtaining their optimum total phenolic content and its relation to antioxidant and antibacterial activities.

Kadir Dler H DH

Food science & nutrition 20210506 7

The main extraction parameters were statistically evaluated for 20 medicinal plants to obtain the optimum conditions for maximum extraction of total phenolic content (TPC) in each plant. Among various extraction parameters, pH, temperature, and concentration at different levels were studied. The results were analyzed using the analysis of variance to achieve the optimum conditions of phenolic extraction for all plants. Also, investigation of the optimum antioxidant (AnOX) activities using DPPH ( ...[more]

PMID: 34262709

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Project description:Nowadays, many studies focus on the potential of bamboo as a source of bioactive compounds and natural antioxidants for nutraceutical, pharmaceutical, and food sources. This study is a pioneering effort to determine the total phenolic content, total flavonoid content and free radical scavenging activity, as well as the phenolic identification and quantification of Bambusa beecheyana. The study was conducted by using ethanol, methanol, and water for solvent extraction by applying cold maceration, Soxhlet, and ultrasonic-assisted extraction techniques. The results showed that Soxhlet and ultrasonic-assisted Bambusa beecheyana culm extracts had an increase in the extract's dry yield (1.13-8.81%) but a constant p-coumaric acid (4) content (0.00035 mg/g) as compared to the extracts from the cold maceration. The ultrasonic-assisted extraction method required only a small amount (250 mL) of solvent to extract the bamboo culms. A significant amount of total phenolics (107.65 ± 0.01 mg GAE/g) and flavonoids (43.89 ± 0.05 mg QE/g) were found in the Soxhlet methanol culm extract. The extract also possessed the most potent antioxidant activity with an IC50 value of 40.43 µg/mL as compared to the positive control, ascorbic acid. The UHPLC-ESI-MS/MS analysis was carried out on the Soxhlet methanol extract, ultrasonic-assisted extract at 40 min, and cold methanol extract. The analysis resulted in the putative identification of a total of five phenolics containing cinnamic acid derivatives. The two cinnamic acid derivatives, p-coumaric acid (4) and 4-methoxycinnamic acid (5), were then used as markers to quantify the concentration of both compounds in all the extracts. Both compounds were not found in the water extracts. These results revealed that the extract from Soxhlet methanol of Bambusa beecheyana could be a potential botanical source of natural antioxidants. This study provides an important chemical composition database for further preclinical research on Bambusa beecheyana.

Dataset Information

Statistical evaluation of main extraction parameters in twenty plant extracts for obtaining their optimum total phenolic content and its relation to antioxidant and antibacterial activities.

Publications

Statistical evaluation of main extraction parameters in twenty plant extracts for obtaining their optimum total phenolic content and its relation to antioxidant and antibacterial activities.

Similar Datasets

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