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Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution.

ABSTRACT: Progress in NMR in general and in biomolecular applications in particular is driven by increasing magnetic-field strengths leading to improved resolution and sensitivity of the NMR spectra. Recently, persistent superconducting magnets at a magnetic field strength (magnetic induction) of 28.2 T corresponding to 1200 MHz proton resonance frequency became commercially available. We present here a collection of high-field NMR spectra of a variety of proteins, including molecular machines, membrane proteins, viral capsids, fibrils and large molecular assemblies. We show this large panel in order to provide an overview over a range of representative systems under study, rather than a single best performing model system. We discuss both carbon-13 and proton-detected experiments, and show that in ¹³C spectra substantially higher numbers of peaks can be resolved compared to 850 MHz while for ¹H spectra the most impressive increase in resolution is observed for aliphatic side-chain resonances.

SUBMITTER: Callon M

PROVIDER: S-EPMC8275511 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution.

Callon Morgane M Malär Alexander A AA Pfister Sara S Římal Václav V Weber Marco E ME Wiegand Thomas T Zehnder Johannes J Chávez Matías M Cadalbert Riccardo R Deb Rajdeep R Däpp Alexander A Fogeron Marie-Laure ML Hunkeler Andreas A Lecoq Lauriane L Torosyan Anahit A Zyla Dawid D Glockshuber Rudolf R Jonas Stefanie S Nassal Michael M Ernst Matthias M Böckmann Anja A Meier Beat H BH

Journal of biomolecular NMR 20210625 6-7

Progress in NMR in general and in biomolecular applications in particular is driven by increasing magnetic-field strengths leading to improved resolution and sensitivity of the NMR spectra. Recently, persistent superconducting magnets at a magnetic field strength (magnetic induction) of 28.2 T corresponding to 1200 MHz proton resonance frequency became commercially available. We present here a collection of high-field NMR spectra of a variety of proteins, including molecular machines, membrane p ...[more]

PMID: 34170475

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Dataset Information

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution.

Publications

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution.

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