Oxidative Damage to RNA is Altered by the Presence of Interacting Proteins or Modified Nucleosides.

ABSTRACT: Oxidative stress triggered by the Fenton reaction (chemical) or UVR exposure (photo) can damage cellular biomolecules including RNA through oxidation of nucleotides. Besides such xenobiotic chemical modifications, RNA also contains several post-transcriptional nucleoside modifications that are installed by enzymes to modulate structure, RNA-protein interactions, and biochemical functions. We examined the extent of oxidative damage to naturally modified RNA which is required for cellular protein synthesis under two different contexts. The extent of oxidative damage is higher when RNA is not associated with proteins, but the degree of damage is lower when the RNA is presented in the form of a ribonucleoprotein complex, such as an intact ribosome. Our studies also indicate that absence of met

SUBMITTER: Estevez M 

PROVIDER: S-EPMC8281250 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature