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Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools.

ABSTRACT: Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools, which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids.

SUBMITTER: Zhu Y

PROVIDER: S-EPMC8291651 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools.

Zhu Yuwei Y Huang Zhiwei Z

National science review 20181129 3

Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summar ...[more]

PMID: 34691893

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