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Coupled sterol synthesis and transport machineries at ER-endocytic contact sites.

ABSTRACT: Sterols are unevenly distributed within cellular membranes. How their biosynthetic and transport machineries are organized to generate heterogeneity is largely unknown. We previously showed that the yeast sterol transporter Osh2 is recruited to endoplasmic reticulum (ER)-endocytic contacts to facilitate actin polymerization. We now find that a subset of sterol biosynthetic enzymes also localizes at these contacts and interacts with Osh2 and the endocytic machinery. Following the sterol dynamics, we show that Osh2 extracts sterols from these subdomains, which we name ERSESs (ER sterol exit sites). Further, we demonstrate that coupling of the sterol synthesis and transport machineries is required for endocytosis in mother cells, but not in daughters, where plasma membrane loading with accessible sterols and endocytosis are linked to secretion.

SUBMITTER: Encinar Del Dedo J

PROVIDER: S-EPMC8294947 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Coupled sterol synthesis and transport machineries at ER-endocytic contact sites.

Encinar Del Dedo Javier J Fernández-Golbano Isabel María IM Pastor Laura L Meler Paula P Ferrer-Orta Cristina C Rebollo Elena E Geli Maria Isabel MI

The Journal of cell biology 20210720 10

Sterols are unevenly distributed within cellular membranes. How their biosynthetic and transport machineries are organized to generate heterogeneity is largely unknown. We previously showed that the yeast sterol transporter Osh2 is recruited to endoplasmic reticulum (ER)-endocytic contacts to facilitate actin polymerization. We now find that a subset of sterol biosynthetic enzymes also localizes at these contacts and interacts with Osh2 and the endocytic machinery. Following the sterol dynamics, ...[more]

PMID: 34283201

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