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Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe.

ABSTRACT: Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a novel small-molecule fluorescent probe based on 9(10H)acridone moiety for nitric oxide sensing. 7,8-Diamino-4-carboxy-10-methyl-9(10H)acridone reacts with NO in aqueous media in the presence of O2, yielding a corresponding triazole derivative with fivefold increased fluorescence intensity. The probe was shown to be capable of nitric oxide sensing in living Jurkat cells.

SUBMITTER: Panfilov M

PROVIDER: S-EPMC8307419 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe.

Panfilov Mikhail M Chernova Darya D Khalfina Irina I Moskalensky Alexander A Vorob'ev Aleksey A

Molecules (Basel, Switzerland) 20210717 14

Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a novel small-molecule fluorescent probe based on 9(10<i>H</i>)acridone moiety for nitric oxide sensing. 7,8-Diamino-4-carboxy-10-methyl-9(10<i>H</i>)acridone reacts with NO in aqueous media in the presenc ...[more]

PMID: 34299615

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