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Sulfamethoxazole-Altered Transcriptomein Green Alga Raphidocelis subcapitata Suggests Inhibition of Translation and DNA Damage Repair.

ABSTRACT: Occurrence of sulfonamide antibiotics has been reported in surface waters with the exposures ranging from < 1 ng L^-1 to approximately 11 μg L^-1, which may exert adverse effects on non-target algal species, inhibiting algal growth and further hindering the delivery of several ecosystem services. Yet the molecular mechanisms of sulfonamide in algae remain undetermined. The aims of the present work are: (1) to test the hypothesis whether sulfamethoxazole (SMX) inhibits the folate biosynthesis in a model green alga Raphidocelis subcapitata; and (2) to explore the effects of SMX at an environmentally relevant concentration on algal health. Here, transcriptomic analysis was applied to investigate the changes at the molecular levels in R. subcapitata treated with SMX at the concentrations of 5 and 300 μg L^-1. After 7-day exposure, the algal density in the 5 μg L^-1 group was not different from that in the controls, whereas a marked reduction of 63% in the high SMX group was identified. Using the adj p < 0.05 and absolute log₂ fold change > 1 as a cutoff, we identified 1 (0 up- and 1 downregulated) and 1,103 (696 up- and 407 downregulated) differentially expressed genes (DEGs) in the 5 and 300 μg L^-1 treatment groups, respectively. This result suggested that SMX at an environmentally relevant exposure may not damage algal health. In the 300 μg L^-1 group, DEGs were primarily enriched in the DNA replication and repair, photosynthesis, and translation pathways. Particularly, the downregulation of base and nucleotide excision repair pathways suggested that SMX may be genotoxic and cause DNA damage in alga. However, the folate biosynthesis pathway was not enriched, suggesting that SMX does not necessarily inhibit the algal growth via its mode of action in bacteria. Taken together, this study revealed the molecular mechanism of action of SMX in algal growth inhibition.

SUBMITTER: Guo J

PROVIDER: S-EPMC8326373 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Nanoparticles (NPs) are causing threats to the environment. Silver NPs (AgNPs) are increasingly used in commercial products and may end up in freshwater ecosystems. The freshwater organisms are vulnerable due to water-borne and dietary exposure to AgNPs. Surface properties play an important role in the fate and behavior of AgNPs in the aquatic environment and their effects on organisms. However, effects of surface properties of AgNPs on organisms are poorly understood. In this study, we explored the effects of AgNPs coated with three different ligands; Tyrosine (T-AgNP), Epigallocatechin gallate (E-AgNP) and Curcumin (C-AgNP) in relation to the toxicity to a key aquatic organism; Daphnia carinata. The study focused on how coatings determine fate of NPs in the medium, mortality, feeding behaviour, bioaccumulation and trophic transfer from the freshwater alga, Raphidocelis subcapitata to daphnids. NP stability tests indicated that T-AgNPs were least stable in the ASTM daphnia medium while C-AgNPs were most stable. 48 h EC50 values of AgNPs to D. carinata were in the order of E-AgNP (19.37 μg L-1) > C-AgNP (21.37 μg L-1) > T-AgNP (49.74 μg L-1) while the 48 h EC50 value of Ag+ ions was 1.21 μg L-1. AgNP contaminated algae significantly decreased the feeding rates of daphnids. However, no significant differences were observed in feeding rates between algae contaminated with differently coated AgNPs. Trophic transfer studies showed that AgNPs were transferred from algae to daphnids. The bioacumulation of AgNPs in algae and the diet-borne bioaccumulation of AgNPs in daphnids varied for differently coated AgNPs. Bioaccumulation of C-AgNPs in algae was 1.5 time higher than T-AgNPs. However, the accumulation of T-AgNPs in daphnids via trophic transfer was 2.6 times higher than T-AgNPs. The knowledge generated from this study enhances the understanding of surface property dependent toxicity, bioaccumulation and trophic transfer of AgNPs in aquatic environments.

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Sulfamethoxazole-Altered Transcriptomein Green Alga Raphidocelis subcapitata Suggests Inhibition of Translation and DNA Damage Repair.

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