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Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1.

ABSTRACT: Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro, providing a versatile platform for imaging-based studies of SOD1.

SUBMITTER: Huang PS

PROVIDER: S-EPMC8330834 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1.

Huang Pei-San PS Wen Meng-Hsuan MH Xie Xihong X Xu An A Lee Dung-Fang DF Chen Tai-Yen TY

Stem cell research 20210606

Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, a ...[more]

PMID: 34118566

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