Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing.
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ABSTRACT: Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.
SUBMITTER: Xu A
PROVIDER: S-EPMC6021017 | biostudies-literature |
REPOSITORIES: biostudies-literature
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