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Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing.

ABSTRACT: Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.

SUBMITTER: Xu A

PROVIDER: S-EPMC6021017 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing.

Xu An A Zhou Ruoji R Tu Jian J Huo Zijun Z Zhu Dandan D Wang Donghui D Gingold Julian A JA Mata Helen H Rao Pulivarthi H PH Liu Mo M Mohamed Alaa M T AMT Kong Celine Shuet Lin CSL Jewell Brittany E BE Xia Weiya W Zhao Ruiying R Hung Mien-Chie MC Lee Dung-Fang DF

Stem cell research 20180427

Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers. ...[more]

PMID: 29730572

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