Identification of genes encoding a novel ABC transporter in Lactobacillus delbrueckii for inulin polymers uptake.

ABSTRACT: Lactobacillus delbrueckii JCM 1002^T grows on highly polymerized inulin-type fructans as its sole carbon source. When it was grown on inulin, a > 10 kb long gene cluster inuABCDEF (Ldb1381-1386) encoding a plausible ABC transporter was suggested to be induced, since a transcriptome analysis revealed that the fourth gene inuD (Ldb1384) was up-regulated most prominently. Although Bacillus subtilis 168 is originally unable to utilize inulin, it became to grow on inulin upon heterologous expression of inuABCDEF. When freshly cultured cells of the recombinant B. subtilis were then densely suspended in buffer containing inulin polymers and incubated, inulin gradually disappeared from the buffer and accumulated in the cells without being degraded, whereas levan-type fructans did not dis

SUBMITTER: Tsujikawa Y 

PROVIDER: S-EPMC8346543 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature