Adaptive immune responses to SARS-CoV-2 in recovered severe COVID-19 patients.

ABSTRACT:

Background

There is an imperative need to determine the durability of adaptive immunity to SARS-CoV-2. We enumerated SARS-CoV-2-reactive CD4⁺ and CD8⁺ T cells targeting S1 and M proteins and measured RBD-specific serum IgG over a period of 2-6 months after symptoms onset in a cohort of subjects who had recovered from severe clinical forms of COVID-19.

Patients and methods

We recruited 58 patients (38 males and 20 females; median age, 62.5 years), who had been hospitalized with bilateral pneumonia, 60% with one or more comorbidities. IgG antibodies binding to SARS-CoV-2 RBD were measured by ELISA. SARS-CoV-2-reactive CD69⁺-expressing-IFNγ-producing-CD4⁺ and CD8⁺ T cells were enumerated in heparinized whole blood by flow c

SUBMITTER: Olea B 

PROVIDER: S-EPMC8349475 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature