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NANOS2 suppresses the cell cycle by repressing mTORC1 activators in embryonic male germ cells.

ABSTRACT: During murine germ cell development, male germ cells enter the mitotically arrested G0 stage, which is an initial step of sexually dimorphic differentiation. The male-specific RNA-binding protein NANOS2 has a key role in suppressing the cell cycle in germ cells. However, the detailed mechanism of how NANOS2 regulates the cell cycle remains unclear. Using single-cell RNA sequencing (scRNA-seq), we extracted the cell cycle state of each germ cell in wild-type and Nanos2-KO testes and revealed that Nanos2 expression starts in mitotic cells and induces mitotic arrest. We identified Rheb, a regulator of mTORC1, and Ptma as possible targets of NANOS2. We propose that repression of the cell cycle is a primary function of NANOS2 and that it is mediated via the suppression of mTORC1 activity through the repression of Rheb in a post-transcriptional manner.

SUBMITTER: Shimada R

PROVIDER: S-EPMC8350546 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells.

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Project description:This SuperSeries is composed of the SubSeries listed below.

2013-02-06 | GSE37720 | GEO

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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